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Objective To investigate the association of chlamydia pneumoniae infection with ankylosing spondylitis (AS).MethodsSerum samples were obtained from 33 AS patients and 22 healthy controls.Enzyme-linked immunosorbent assay (ELISA) was applied to mearsure serum anti-Chlamydia pneumoniae antibodies (IgM/IgG),while immunofluorescence assay (IFA) was used to detect Chlamydia pneumoniae LPS antigen,and polymerase chain reaction (PCR) was used to amplify Chlamydia pneumoniae DNA in peripheral blood cells. Immunohistochemistical technique was applied to examine Chlamydia pneumoniae LPS antigen in synovial tissue from another 9 AS patients who received total hip replacement and 13 patients with comminuted femoral fractures.ResultsThe positive rates of Chlamydia pneumoniae IgM,LPS antigen and chlamydia pneumoniae DNA were higher in AS patients than those in healthy controls (78.8％ vs 22.7％,x2 =16.867,P =0.000; 66.7％ vs 31.8％,x2 =6.431,P =0.011; 33.3％ vs 9.1％,x2 =4.298,P =0.038).Chlamydia pneumoniae DNA positive rate was correlated with erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) levels (Z =-2.774 and -2.829,P =0.004).In synovial tissues,chlamydial LPS-containing inflammatory cells were observed in 77.8％(7/9) AS patients,while those in fracture patients was 30.8％ ( 4/13 ) ( P =0.08 ).Conclusion Chlamydia pneumoniae infection is common in blood circulation and joint cavity of AS patients and may be associated with the pathogenesis of AS.
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BACKGROUND:Femoral shaft fracture is common in children Various methods of treatment can be used successfully,depending On the age of the child and the type of fracture.OBJECTIVE:To evaluate the feasibility of adult reconstruction plate of humeral shaft for pediatric femoral shaft fracture.METHODS:A total of 32 children with femoral shaft fracture were treated with adult reconstruction plate of humeral shaft in First Department of Orthopedics,Fuzhou General Hospital of Fujian Medical University,including 20 males and 12 females aged 6.7years(5-8 years).All patients were treated with incision reduction and adult reconstruction plate of humeral shaft.The incision length should be made as short as possible according to the fracture type The result of surgery was determined using clinical and radiographic examinations The pain condition was evaluated using visual analog scale method before and 3 days following surgery.RESULTS AND CONCLUSION:The patients were followed up for 1-2 years(average 1.5 years).All fractures were healed 1-2months postoperatively,and the internal fixator was removed 4-8 months postoperatively.The affected limb was shortened0.6-1.5 cm(average 1.1 cm)in 3 cases.Overgrowth was obsewed in the other patients by 0.3-1.2 cm The average overgrowth length was(0.64±0.312)cm in 29 patients.At 3 days postoperatiVely,the mean subjective pain was significantly reduced,and range of motion was improved compared with the day before surgery There was no infection or implant displacement or re-fracture.It is feasible to use adult reconstruction plate of humeral shaft for paediatric femoral shaft fracture.
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Objective To observe the inhibition effect of recombinant human monocyte chemoattractant protein－ 1(MCP－ 1) on implantation and growth of human osteosarcoma cells. Methods The method of protein fusion was used for the expression of MCP－ 1 in b.coli and then the MCP－ 1 was extracted and purified. Fifty nude mice were divided into 10 groups. For A1－ 4 groups, the 5 mice in each group were locally injected with doses of 1? g, 10? g, 100? g, 1 mg of MCP－ 1 at the same time when 4.4? 106 osteosarcoma cells were implanted in vivo. For B0－ 4 groups, 5 mice in each group, the injection were given 2 weeks later when there was the formation of the tumor mass at doses of 0? g(0.2 ml normal saline), 1? g, 10? g, 100? g, 1 mg of MCP－ 1 every other day. Five mice in group C were injected with dose of 0.2 ml NS as control. Results Implantation of osteosarcoma cells were completely prevented among mice of group A2－ 4, tumor inhibiting effect even in group A1 and the rate of tumor inhibition was 69.69％ . AKP values in mice of group A1－ 4 were much lower than those of group B0(P